Journal: Journal of experimental and theoretical analyses

Article Title: The Application of Fluorescence Anisotropy for Viscosity Measurements of Small Volume Biological Analytes

doi: 10.3390/jeta1020007

Figure Lengend Snippet: Time-resolved anisotropy measurements of EGFP in different sample volume sizes. Rotational correlation times were derived from time-resolved fluorescence anisotropy measurements of EGFP at different viscosities. The measurements collected with two different analyte volumes (12 μL and 100 μL); ns—not significant; n = 3–4; error bars illustrate standard deviation (SD).

Article Snippet: The fluorescent dyes used in this study were fluorescein disodium salt from Alfa Aesar (Ward Hill, MA, USA) and recombinant EGFP from Novus Biological (NBP1–99915, Centennial, CO, USA) with N- and C-terminal His tags.

Techniques: Derivative Assay, Fluorescence, Standard Deviation

Journal: Journal of experimental and theoretical analyses

Article Title: The Application of Fluorescence Anisotropy for Viscosity Measurements of Small Volume Biological Analytes

doi: 10.3390/jeta1020007

Figure Lengend Snippet: Time-resolved anisotropy measurements of fluorescein end EGFP in 100 μL samples 1.02 cP viscosity standard. Rotational correlation times are specific to the experimental probe used; n = 3–4; error bars illustrate SD.

Article Snippet: The fluorescent dyes used in this study were fluorescein disodium salt from Alfa Aesar (Ward Hill, MA, USA) and recombinant EGFP from Novus Biological (NBP1–99915, Centennial, CO, USA) with N- and C-terminal His tags.

Techniques: Viscosity

Journal: Journal of experimental and theoretical analyses

Article Title: The Application of Fluorescence Anisotropy for Viscosity Measurements of Small Volume Biological Analytes

doi: 10.3390/jeta1020007

Figure Lengend Snippet: The response of EGFP rotational correlation times in a biological sample mimetic. ( A )—comparison of rotational correlation times in samples with or without FBS; ( B )—evaluation of freeze–thaw effects rotational correlation times in samples with FBS. A two-way ANOVA with Sidak post-test was used to compare the data. ns—not significant; n = 3–4; error bars illustrate SD.

Article Snippet: The fluorescent dyes used in this study were fluorescein disodium salt from Alfa Aesar (Ward Hill, MA, USA) and recombinant EGFP from Novus Biological (NBP1–99915, Centennial, CO, USA) with N- and C-terminal His tags.

Techniques: Comparison

Journal: Annals of Clinical and Translational Neurology

Article Title: Protein‐extending ACTN2 frameshift variants cause variable myopathy phenotypes by protein aggregation

doi: 10.1002/acn3.52154

Figure Lengend Snippet: List of ACTN2 frameshift variants identified in the described patients, and variants curated from literature and databases.

Article Snippet: Recombinant eGFP‐tagged c.2567del (p.Pro856Argfs*45), c.2569_2570insC (p.Asp857Alafs*83), and c.392T>C (p.Leu131Pro) single mutant ACTN2 constructs were generated by GenScript (New Jersey, USA).

Techniques: Sequencing

Journal: Annals of Clinical and Translational Neurology

Article Title: Protein‐extending ACTN2 frameshift variants cause variable myopathy phenotypes by protein aggregation

doi: 10.1002/acn3.52154

Figure Lengend Snippet: Clinical features of patients with ACTN2 variants reported in this study, and patients with protein‐extending ACTN2 frameshift variants previously reported in literature.

Article Snippet: Recombinant eGFP‐tagged c.2567del (p.Pro856Argfs*45), c.2569_2570insC (p.Asp857Alafs*83), and c.392T>C (p.Leu131Pro) single mutant ACTN2 constructs were generated by GenScript (New Jersey, USA).

Techniques: